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RNase III cleaves long double-stranded RNA (dsRNA) into short 12-30 base dsRNAs containing a 5'-PO4
The following amounts are for a single 20 μL reaction
Companion Product: 10X Capping Buffer
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10X Capping Buffer GMP level:Non-GMP RNase III cleaves long double-strandedThe 10X Capping Buffer is a buffer solution used in conjunction with the Faustovirus capping enzyme, Vaccinia Capping Enzyme, mRNA Cap 2' O Methyltransferase. Related Buffer: S adenosylmethionine (SAM) Inside with: 500mM Tris HCl, pH 8. 0, 50mM KCl, 10mM MgCl2, 10mM DTT. Recommended Storage: 20 For detailed oprating instructions, please refer to the enzyme manual.
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