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allow it to equilibrate to room temperature before use
centrifugation for 5 min to collect
1 Calculate the total volume of working fluid required: 100ul/ Hole × Number of holes
122(4): 865–869
Rat CCKAR ELISA Kit Size:96T allow it to equilibrate toProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and